解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Bladder smooth muscle contraction is mediated by both direct calcium entry through the cell membrane, and by calcium induced calcium release (CICR) from the sarcoplasmic reticulum (SR) storage sites. Ryanodine is a neutral plant alkaloid which binds to an ion channel located on the SR membrane. Its effects in cardiac ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00944078
更新日期:1994-08-31 00:00:00
abstract::Iron ions play a central role in .OH radicals formation and induction of oxidative stress in living organisms. Iron-catalyzed .OH radical formation degrades deoxyribose to thiobarbituric acid reactive substances (TBA-RS). This paper analyzes kinetic properties of the Fe(III)-EDTA-catalyzed deoxyribose degradation in t...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926041
更新日期:1994-08-17 00:00:00
abstract::The effect of beta-alanyl-L-histidinato zinc (AHZ) on protein components in osteoblastic MC3T3-E1 cells was investigated. Cells were cultured for 3 days at 37 degrees C in CO2 incubator in plastic dishes containing alpha-modified minimum essential medium supplemented with 10% fetal bovine serum. After the cultures, th...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926077
更新日期:1994-07-27 00:00:00
abstract::The involvement of a hypocalcemic hormone calcitonin (CT) in the expression of hepatic Ca(2+)-binding protein regucalcin mRNA was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb). A single oral administration of calcium chloride (100...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00931603
更新日期:1994-07-13 00:00:00
abstract::Cytosolic purine nucleoside phosphorylase (PNPase) is a well known, and described enzyme which exists in a variety of organisms, both procaryotic and eucaryotic. More recently this enzyme was found in bovine liver mitochondria. The mitochondrial purine nucleoside phosphorylase was purified 63 fold and has a molecular ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926515
更新日期:1994-06-29 00:00:00
abstract::The fact that smooth muscle exists in almost every hollow organ and is involved in a large number of disease states has led to a vast increase in smooth muscle research, covering areas from testing response to antagonists and agonists to measuring the molecular force generated by a single actin filament. Yet, the exac...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00925956
更新日期:1994-06-15 00:00:00
abstract::The last decade has seen the rapid development of research investigating the molecular mechanisms whereby hormones, peptide growth factors and cytokines regulate cell metabolism, differentiation and proliferation. One general signalling mechanism used to transfer the information delivered by agonists into appropriate ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00925964
更新日期:1994-06-15 00:00:00
abstract::This article is a review on the organization and function of myofibrillar creatine kinase in striated muscle. The first part describes myofibrillar creatine kinase as an integral structural part of the complex organization of myofibrils in striated muscle. The second part considers the intrinsic biochemical and mechan...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF01267952
更新日期:1994-04-01 00:00:00
abstract::Human cardiomyopathy has been extensively studied in the last decade, and knowledge of the functional and structural alterations of the heart has grown. However, understanding of the pathogenesis has come mostly from experimental studies. A number of work have been designed to elucidate if alterations of the contracti...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-2612-4_19
更新日期:1994-04-01 00:00:00
abstract::In order to compare the role of the Ca(2+)-receptive protein (troponin), in the characteristic myofibrillar contractile response of chicken fast and slow skeletal muscles, the troponin in both kinds of myofibrils were partially exchanged, under slightly acidic conditions. The Ca(2+)- or Sr(2+)-activation of the ATPase...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926926
更新日期:1994-03-30 00:00:00
abstract::To define metabolic influences on cardiac myosin expression and sarcoplasmic reticulum (SR) Ca(2+)-stimulated ATPase streptozotocin-diabetic rats were treated for 9-10 wk with etomoxir, an inhibitor of carnitine palmitoyl transferase I (CPT-1) and fatty acid synthesis, or an antilipolytic drug, acipimox. Etomoxir redu...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925676
更新日期:1994-03-16 00:00:00
abstract::It has been recently hypothesized that in PIH a placental oxidant-antioxidant imbalance might cause the release of lipoperoxidation products into the circulation, with subsequent damage of endothelial cell membranes. In this hypothesis the endothelial cell and further increase in circulating lipoperoxide levels, which...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925951
更新日期:1994-02-23 00:00:00
abstract::Carnitine is associated with lipid synthesis and its deficiency may lead to cardiomegaly with parenchymal lipid in the heart, kidney and liver. In our study we found that pretreatment of peripheral blood mononuclear cells (PBMC) with serial dilutions of L-Carnitine (100 micrograms/ml-1 pg/ml) inhibits, in a dose-depen...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01075718
更新日期:1994-02-09 00:00:00
abstract::A high molecular weight protein has been isolated as a major polypeptide comprising 85% of the total extractable proteins in the fruit pericarp of the plant Impatiens balsamina. The protein has been purified to homogeneity following fractionation of the crude cell supernatant with ammonium sulphate, chromatography on ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01457392
更新日期:1994-01-26 00:00:00
abstract::Partial obstruction of the rabbit bladder outlet induces a rapid hypertrophy characterized by increased bladder mass, increased smooth muscle content, and increased collagen deposition. In addition, partial outlet obstruction induces decreased contractile responses to both field stimulation and postsynaptic receptor s...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01084267
更新日期:1994-01-12 00:00:00
abstract::Oxygen is a diradical and because of its unique electronic configuration, it has the potential to form strong oxidants (e.g. superoxide radical, hydrogen peroxide and hydroxyl radical) called oxygen free radicals or partially reduced forms of oxygen (PRFO). These highly reactive oxygen species can cause cellular injur...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00926366
更新日期:1993-12-22 00:00:00
abstract::Tripeptide aminopeptidase (EC 3.4.11.4) was purified from bovine dental follicles by a series of chromatographies. Purified enzyme had a specific activity of 59.5 units per mg protein with L-prolyl-glycylglycine as substrate. The pH optimum was 8.0. The purified native enzyme had a Mr of 230,000 and was shown to be a ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926579
更新日期:1993-12-08 00:00:00
abstract::Casein kinase II is a protein serine/threonine kinase that is ubiquitously distributed in eukaryotes. Molecular cloning studies and protein sequence analysis of purified proteins have demonstrated the existence of two related, but distinct, isoenzymic forms of its catalytic subunit in mammals and birds. At present, th...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF01076770
更新日期:1993-11-01 00:00:00
abstract::Limited proteolysis of rabbit muscle phosphorylase kinase catalyzed by chymotrypsin generates a 33 kD product whose kinase activity is independent of both calcium and pH over the range of 6.8 to 8.3 (Malencik, D.A. & Fischer, E.H. Calcium and Cell Function III: 161-188, 1982). This active preparation consists of three...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01076755
更新日期:1993-11-01 00:00:00
abstract::Catalase plays a major role in the protection of tissues from toxic effects of H2O2 and partially reduced oxygen species. In the present study catalase was extracted and purified 330-fold from goat lung by acetone fractionation and successive chromatographies on DEAE-cellulose, Sephadex G-200, Blue Sepharose CL-6B and...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925690
更新日期:1993-09-22 00:00:00
abstract::A monocyte chemotactic protein (MCP-1) is thought to play a major role in recruiting monocytes to the vascular endothelium where the adherence of monocytes is one of the earliest events in atherogenesis. We cloned MCP-1 cDNA from a lambda gt 11 cDNA library constructed from human aortic endothelial mRNA to test whethe...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01772208
更新日期:1993-09-08 00:00:00
abstract::Pregna-D'-pentaranes (pentaranes) are modified progesterones with demonstrable progestational activity and contraceptive effect. We have examined the steroid binding characteristics of the two newly synthesized progesterone analogs, Pentarane A (16 alpha, 17 alpha-cyclohexanoprogesterone) and Pentarane B (6 alpha-meth...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00936444
更新日期:1993-08-25 00:00:00
abstract::Recent studies indicate that the mucosa of the urinary bladder may play a major role in the maintenance of normal bladder function. The mucosal surface of the urinary bladder serves as a protective layer against the irritative solutes found in the urine. The integrity of this barrier can be broken by overdistension, a...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926832
更新日期:1993-08-11 00:00:00
abstract::Those genes which are transcribed by RNA polymerase III continue to give surprising results with respect to their cis-acting elements and transacting factors. As a result, a broader view of class III promoters has emerged and the internal promoters are not universal in classical polymerase III genes. The involvement o...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF01096385
更新日期:1993-07-07 00:00:00
abstract::A relation between fatty acid oxidation capacity and cytosolic FABP content was found in heart and various muscles of the rat. Other tissues do not show such a relation, since they are involved in more or other pathways of fatty acid metabolism. At postnatal development FABP content and fatty acid oxidation capacity r...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01076480
更新日期:1993-06-09 00:00:00
abstract::Ischemia of the heart is accompanied by the tissue accumulation of long-chain fatty acids and their metabolic derivatives such as beta-hydroxy fatty acids and fatty acyl-CoA and acyl-L-carnitine esters. These substances might be detrimental for proper myocardial function. Previously, it has been suggested that intrace...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF01076489
更新日期:1993-06-09 00:00:00
abstract::Fatty acid-binding proteins (FABP) are abundant cytosolic proteins whose levels is responsive to nutritional, endocrine, and a variety of pathological states. Although FABPs have been investigated in vitro for several decades, little is known of their physiological function. Liver L-FABP binds both fatty acids and cho...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01076477
更新日期:1993-06-09 00:00:00
abstract::In an attempt to understand the functional role of individual transcription factors, we have carried out S1 nuclease analyses of in vitro synthesized RNA using partially purified HeLa cell factors and truncated 5S and tDNA. The results indicate that faithful initiation of 5S gene transcription depends on the presence ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925972
更新日期:1993-04-21 00:00:00
abstract::The expression of constitutive HSP-70 in the urinary bladder was determined by SDS-PAGE and western blotting using a mouse monoclonal antibody against HSP-70. The western blot analysis showed that the mouse anti-HSP-70 cross-reacted with a 70 kDa protein present in the extracts of the urinary bladder muscle and mucosa...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00928695
更新日期:1993-04-07 00:00:00
abstract::This paper reviews characteristics of microsomal membrane structure; long chain fatty acids, acyl CoA derivatives, retinoids and the microsomal formation of acyl CoA derivatives and retinyl esters. It is analyzed how the movement of these molecules at the intracellular level is affected by their respective binding pro...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00926080
更新日期:1993-03-24 00:00:00
abstract::We have tested the hypothesis that activation of the insulin receptor tyrosine kinase is due to autophosphorylation of tyrosines 1146, 1150 and 1151 within a putative autoinhibitory domain. A synthetic peptide corresponding to residues 1134-1162, with tyrosines substituted by alanine or phenylalanine, of the insulin r...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926082
更新日期:1993-03-24 00:00:00
abstract::Administration of nifedipine to mice over a period of six months caused a significant (p < 0.05) decrease in neutrophilic functions viz superoxide generation, coupled to NADPH oxidase activity as well as NADPH production by HMP shunt. Properties like chemotaxis and phagocytosis showed a similar decrease. From this stu...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925987
更新日期:1993-03-10 00:00:00
abstract::Photoreactive probes for the hydrophobic pocket of the liver fatty acid-binding protein, 11-(5'-azido-salicylamido)-undecanoic acid (5' ASU) and its acetyl ester (Ac5' ASU), were synthesized and their interaction with the protein was assessed. Fatty acid-binding proteins are closely related proteins which are abundant...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925980
更新日期:1993-03-10 00:00:00
abstract::The characterization of mutations in Japanese patients with lipidosis, particularly in metachromatic leukodystrophy (MLD) and Gaucher disease has been studied in detail. Metachromatic leukodystrophy is characterized by an accumulation of sulfatide in nervous tissues and kidney due to a deficiency of arylsulfatase A (A...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926869
更新日期:1993-02-17 00:00:00
abstract::We measured the relative sliding velocity of cardiomyopathic hamster cardiac myosin on actin cables by using an in vitro motility assay system. We also investigated the relationship between the velocity and both myosin isozyme content and ATPase activity. Cardiac myosin was obtained from cardiomyopathic hamsters (BIO ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926857
更新日期:1993-02-17 00:00:00
abstract::Cardiac hypertrophy in rats was produced by aortic banding for 6 weeks and regression of hypertrophy in these experimental animals was induced by administration of angiotensin converting enzyme inhibitor, enalapril (10 mg/kg/day) for 6 weeks. The left ventricular muscle mass and systolic pressure were decrease upon tr...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926849
更新日期:1993-02-17 00:00:00
abstract::Effects of iloprost, which is a stable prostacyclin analogue, on the ischemic myocardium were examined in the open-chest dog heart in terms of biochemical parameters. Ischemia was initiated by ligating the left anterior descending coronary artery. When the coronary artery was ligated for 3 min, the levels or glycogen,...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926864
更新日期:1993-02-17 00:00:00
abstract::The present study examines the time dependent effects of n-6 and n-3 polyunsaturated fatty acids on liver microsomal lipid metabolism in FVB mice fed a diet supplemented with a mixture of free fatty acids (mainly 18:3n-6 and 20:5n-3) at 25 mg/g diet. Significant changes in the fatty acid composition of total liver and...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00299394
更新日期:1992-12-16 00:00:00
abstract::The antineoplastic drug estramustine is an adduct of estradiol and nor-nitrogen mustard. It has been shown that this drug interferes with microtubule assembly, an effect mediated by estramustine interaction with microtubule-associated proteins (MAPs). In the present report we demonstrate that estramustine and the phos...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00230756
更新日期:1992-11-18 00:00:00
abstract::We report here a study of the incorporation and metabolism of various long chain fatty acids in SK-Hep-1 cultured hepatoma cells. Medium supplementation with radiolabelled palmitic, stearic, linoleic, alpha-linolenic and eicosa-8,11,14-trienoic acids (1 microM, 24 H) resulted in an active uptake of each of these precu...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00230749
更新日期:1992-11-18 00:00:00